Genetic Variations in the Ace1, Ace2, and Tmprss2 Sars-Cov2 Receptor Genes in the Members of a Filipino Community
Date of Award
12-1-2023
Document Type
Thesis
Degree Name
Master of Science in Biology
First Advisor
Vivian A. Panes, PhD
Abstract
In 2020, the coronavirus disease-19 (COVID-19) was declared a global pandemic. It has led to millions of deaths in the Philippines and even worldwide. Despite its prevalence, COVID-19 patients manifested varying levels of disease severity and different responses to treatment methods. Therefore, risk factors for COVID-19 susceptibility and severity are being explored. These risk factors include genetic variants, sex, age, ethnicity, lifestyle, and comorbidities. In particular, this study explored the genetic variants of ACE1, ACE2, and TMPRSS2, because these genes play crucial roles in SARS-CoV2 infection. This study aimed to identify the presence of ACE1 rs4343, ACE2 rs228566, and TMPRSS2 rs12329760 in Filipino subjects, and to correlate these variants with different risk factors that affect COVID-19 susceptibility and severity. A total of 42 participants were recruited for the study. The variants were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, which was confirmed through DNA sequencing. The results indicated that the G/G genotype of ACE2 rs228566 was associated with male samples, whereas the G/A genotype was associated with female samples. The G/G genotype also correlated with the presence of at least one comorbidity. The A/A genotype was correlated with previous COVID-19 infections in the current study involving Filipinos. Finally, the T allele of TMPRSS2 rs12329760 is associated with blood type A. The results of this study provide valuable information regarding the genetic profile of COVID-19-related gene variants in the Filipino group. This study is the first to report the genetic variants of ACE1, ACE2, and TMPRSS2 in the Philippines. These findings underscore the significance of genetic variants in understanding the heterogeneity of COVID-19 and the potential of these single nucleotide polymorphisms (SNPs) as targets for future research
Recommended Citation
Sy, Jenica Clarisse Y., (2023). Genetic Variations in the Ace1, Ace2, and Tmprss2 Sars-Cov2 Receptor Genes in the Members of a Filipino Community. Archīum.ATENEO.
https://archium.ateneo.edu/theses-dissertations/849
