Cellulase Activity of Fungal Isolated and Phanerochaete Chrysosporium Co-Culture Isolates Using Rice Straw as Substrate

Date of Award

2020

Document Type

Thesis

Degree Name

Master of Science in Biology

Department

Biology

First Advisor

Dr.rer.nat. Crisanto M. Lopez

Abstract

Rice straw, one of the most abundant lignocellulosic waste is rich in cellulose, which makes it a favorable substrate for cellulases. Phanerochaete chrysosporium is a white-rot basidiomycete that is best known for its ligninolytic enzyme system, however, there were only few reports regarding its cellulase activity. This study evaluated the compatibility of unknown fungal isolates (UFIs) collected from decaying woods with P. chrysosporium as well as their cellulase activities and glucose yields of their monoculture and co-culture isolates. Qualitative and quantitative screening tests revealed that the P. chrysosporium co-culture SP 6.1 + PC, obtained the highest cellulase activity and glucose yield. Moreover, SP 6.1 + PC also exhibited the highest level of significant difference (p<0.0001) compared to its monoculture isolates. The macroscopic colony morphology and molecular methods revealed that all UFIs are fungal species from Phylum Ascomycota. Specifically, the UFIs SP 3.2, SP 5.1, SP 6.1, SP 9, and SP 12 were Aspergillus clavatophorus, Meyerozyma guilliermondii, Xylaria grammica, Penicillium shearii, and Hypoxylon sp., respectively. The most promising co-culture isolate combination was revealed as P. chrysosporium and X. grammica. Surprisingly, there were only few reports on the enzyme profiles of the fungal isolates utilized. The present study, therefore, demonstrated the potential of P. chrysosporium co-culture of X. grammica as efficient cellulase producers utilizing rice straw as a substrate.

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