Cyclodextrin Metal-Organic Framework (CD-MOF) synthesis and its application in loading bio-active molecules

Date of Award

2017

Document Type

Thesis

Degree Name

Master of Science in Chemistry

Department

Chemistry

First Advisor

Yu, Gilbert U., Ph.D.

Abstract

Metal-organic frameworks (MOFs) represent an extensive class of porous materials where coordination of metal ions with organic ligands give rise to networks containing potential voids. In the study, colorless cubic crystals of a 'green' cyclodextrin metal-organic framework (CD-MOF) were synthesized and its potential to load bio-active molecules such as drugs and enzymes was investigated. Busulfan, an anti-cancer drug widely used to treat chronic myelogenous leukemia was used as test drug while trypsin and lipase were used as model enzymes. The CD-MOFs were prepared by dissolving alkali metal salts and ?-cyclodextrin in water followed by vapor diffusion in varying solvents (methanol, ethanol, acetone and tetrahydrofuran) for several days. Crystals were activated via dichloromethane dispersion and in vacuo drying. Slow vapor diffusion with ethanol was found to give the highest yield and fastest crystallization time among all the solvents used. CD-MOFs synthesized using ethanol were characterized to be cubic crystals with sizes ranging from 1000-100m that are stable up to 250C. Loading of busulfan (%S) onto the CDMOFs and co-crystallization with trypsin (%N) were both successful as confirmed by elemental analysis. Loading studies of busulfan performed using 1H-NMR spectroscopy confirmed about 5 wt% loading in CD-MOFs.

Comments

The C4.P477 2017

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