Comparative Toxicity, Phytochemistry, Angiogenic, Anticancer, and Regenerative Potentials of Selected Philippine Medicinal Plants
Date of Award
12-1-2023
Document Type
Dissertation
Degree Name
Doctor of Philosophy in Chemistry (Standard Program)
First Advisor
Nina Rosario L.Rojas, PhD
Abstract
As a contribution to the continuing effort in searching for safe and effective herbal medicines, 53 Philippine medicinal plants were investigated for scientific support for the plants’ medicinal claims. We used four-model systems: 1 model organism (brine shrimp lethality assay, BSLA - Artemia sp.) and 3 cell-based models (colorectal cancer cell line HCT 116, human umbilical vein endothelial cells HUVEC and human bone marrow mesenchymal cells hBM-MSC).
The brine shrimp lethality assay was used to estimate general toxicity of the extracts. From the results, a modified classification scheme was proposed: highly toxic, LC50 ≤ 12; moderately toxic,12 < LC50 ≤ 100; mildly toxic,100 < LC50 ≤ 500; essentially nontoxic, 500 < LC50. Anticancer potential was inferred from the cytotoxicity effects of the plant extracts on HCT 116. On the other hand, regenerative potential was inferred from the promotion of proliferation on stem cells hBM-MSCs. Cardiovascular potential was inferred from angiogenic effects of the extracts on HUVEC.
Finally, from the four-model systems adopted, extracts from Abelmoschus esculentus, Allium cepa and Ipomea batatas showed regenerative potential on hBM-MSCs and HUVECs versus their inhibitory activity on HCT 116. This suggests the regenerative potential of these plants extracts with a relatively low risk of promoting cancer. Lastly, qualitative chemical profile using 1H-NMR spectra revealed the presence of compounds that contains, nitrogen based on the exchangeable protons and possible rings and unsaturated carbons. Further study is encouraged to determine the compounds responsible for the proliferative and inhibitory effects, as well as elucidation of the mechanisms of action.
Recommended Citation
Pascual, Lydia C., (2023). Comparative Toxicity, Phytochemistry, Angiogenic, Anticancer, and Regenerative Potentials of Selected Philippine Medicinal Plants. Archīum.ATENEO.
https://archium.ateneo.edu/theses-dissertations/1030
