DNA-based lateral flow strip biosensors for detection of shrimp pathogens

Date of Award

2015

Document Type

Thesis

Department

Chemistry

First Advisor

Rojas, Nina Rosario L., Ph.D.

Abstract

Shrimp diseases are a major risk in shrimp aquaculture industry and early detection of these diseases such as using a point-of-care diagnostic can reduce this risk. Major threats include the acute hepatopancreatic necrosis disease (AHPND) and the white spot syndrome disease caused by white spot syndrome virus (WSSV). In this study, different designs of DNA-based lateral flow strip biosensor (LFSB) were investigated for detection of the WSSV and AHPND pathogens. First, competitive-based LFSB was developed for detection of WSSV viral genome in infected shrimp tissue samples. WSSV-infected shrimp samples were tested and yielded positive results up to 1:100 dilution, while DNA from WSSV-negative shrimp samples yielded negative test outcome. The diagnostic sensitivity and specificity of the strips were estimated to be 92.6% and 92.9%, respectively. For the second design, sandwich-based LFSB was used to detect synthetic DNA targets as mock for AHPND bacterial DNA and also WSSV viral genome. Both the single- and multiple-analyte versions have analytical sensitivity of 10-7 M of synthetic DNA. Overall, this study demonstrates the superiority of genome-based detection over that of antibody-based LFSB in terms of fast adaptability for emerging pathogens and easy design of the detector probes.

Comments

The C4.B246 2015

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