In this study, viability of employing the waste treatment technique for industrial application was conducted by setting up a phytoremediation protocol for field application where heavy metal (Arsenic and Copper) contaminated adsorbents, i.e. coco peat and nanofiber membrane, used for the treatment of wastewater were also included. An arsenic hyperaccumulator, Pityrogramma calomelanos was used for the treatment of mine wastes-tailings, coco peat and nanofiber membrane. Phytoremediation procedure was established by investigating planting parameters namely ; plot height - (9 cm and 15 cm) and planting distance- (15 cm and 20 cm) for a period of five months. Translocation factor and % uptake of As and Cu by ferns were measured. According to the results, % uptake of As and Cu by P. Calomelanos was 0.16 % and 0.01 % and translocation factor of As and Cu was 6.78 and 0.04, respectively. Plant growth factors (root length, frond height and dry weight) in tailings mixture plant boxes and control soil were also determined. Accordingly, P. calomelanos grew well in tailings mixture without the symptom of phytotoxicity. Therefore, P. calomelanos can be used for the phytoremediation of the said heavy metal contaminated mine wastes. Based on this study, 15 cm plot height and 20 cm plant distance were suitable for field experiment although long term research data is required. The overall protocol for the treatment of mine wastes as field experiment was proposed based on the results.
Win, Z. C., Diaz, L. J. L., Perez, T. R., & Nakasaki, K. (2020). Phytoremediation of heavy metal contaminated wastes from small-scale gold mining using Pityrogramma calomelanos. E3S Web of Conferences, 148, 05007. https://doi.org/10.1051/e3sconf/202014805007