Radiation-sterilization at 25 and 35 kGy of freeze-dried human amniotic membranes caused degradative effects in the biologic dressing. The decrease in pH and increase in UV absorption showed that radiation may have caused possible radiolytic changes in this biomaterial. Total nitrogen content, tensile strength, and [H3]water-retention capacity of the irradiated membranes remained invariable. Molecular topography analysis by atomic force microscopy showed radiation-induced defrayment of the collagen fibers, the major structural protein in amnion.
Abad, L., Aliganga, A., De Guzman, Z., Deocaris, C., Deocaris, C., Enriquez, E., Ignacio, L, Tangonan, M., Tolentino, M. (2005). Radiolytic Damage to Freeze-dried Human Amniotic Membrane. Philippine Journal of Science, 134 (1), 45-50.