Preparation and Binding Evaluation of Histamine-Imprinted Microspheres via Conventional Thermal and RAFT-Mediated Free-Radical Polymerization

Authors

Edwin F. Romano Jr., Ateneo de Manila University
Regina C. So, Ateneo de Manila University
Scott W. Donne, University of Newcastle
Clovia I. Holdsworth, University of Newcastle

Document Type

Article

Publication Date

10-6-2016

Abstract

Elevated histamine (HTM) levels are closely linked to food poisoning as well as to pathophysiological allergic diseases. In this study, HTM-imprinted, solution-processable microspheres were prepared via high-dilution conventional thermal polymerization (CTP) and controlled radical polymerization (CRP) using ethylene glycol dimethacrylate (80 or 90 wt %) and methacrylic acid at 60 °C in acetonitrile and evaluated as recognition materials for sensing applications. The polymers were selective to HTM in binding studies, cross-rebinding, and competitive binding assays against the HTM analogues histidine, imidazole, and tryptamine. The selective binding capacity was significantly higher with CTP-80 (on the basis of mass: 21.0 μmol/g and surface area: 8.08 × 10^–2 μmol/m²) than that with both CTP-90 (8.47 μmol/g, 4.49 × 10^–2 μmol/m²) and CRP-80 (9.00 μmol/g, 1.19 × 10^–2 μmol/m²).

Recommended Citation

Romano, E. F., So, R. C., Donne, S. W., & Holdsworth, C. I. (2016). Preparation and binding evaluation of histamine-imprinted microspheres via conventional thermal and raft-mediated free-radical polymerization. ACS Omega, 1(4), 518–531. https://doi.org/10.1021/acsomega.6b00144