Antibiotic and anti-quorum sensing activity of lysates from bioluminescent symbiotic bacteria from selected marine and freshwater organisms

Date of Award

2015

Document Type

Thesis

Department

Biology

First Advisor

Ragaza, Janice A., Ph.D.

Abstract

In this study, 13 bioluminescent bacteria designated here as strains ADMU-UE-01 to 13, were isolated from marine and freshwater hosts. Six (6) pure cultures were subsequently obtained and screened, five (5) of which were from marine organisms, specifically BLB-ADMU-UE-01 (Johnius belangerii/ croaker), BLB-ADMU-UE-02 (Sillago analis/ golden-lined sillago), BLB-ADMU-UE-06 (Variola louti/ lapu-lapu), BLB-ADMU-UE-10 (Strombus variabilis/ variable conch ), BLB-ADMU-UE-12 (Cistopus indicus/ octopus) and only one (1) from a freshwater organism, BLB-ADMU-UE-04 (Chanos chanos/ milkfish), yielding Membrane Fraction (MF) with anti-quorum sensing activity against Pseudomonas aeruginosa based on the statistical results (p < 0.05). This study also indicated that the MF of the strain BLB ADMU-UE-10 has significant difference (p < 0.05) from the 0.15 mg/mL caffeine (positive control), showing that it has a very high anti-quorum sensing potential. Despite the differences in their hosts, the bacterial isolates shared evolutionary relationship with commonly known bioluminescent genera, using their respective 16S rRNA gene sequences; the BLB-ADMU-UE-01, BLB-ADMU-UE-02, BLB-ADMU-UE-04, BLB-ADMU-UE-06, and BLB-ADMU-UE-12 belonged to the genus Photobacterium (P. leiognathi and P. mandapamensis, 99-100% identity) while the BLB-ADMU-UE-05 and BLB-ADMU-UE-10 belonged to genus Vibrio (V. harveyi and V. campbelli, 99% identity).

Comments

The B5.A67 2015

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